-
FEBS Letters Nov 2000Bordetella hinzii has recently been isolated from immunocompromised human hosts. The polysaccharides isolated from its endotoxin (lipopolysaccharide, LPS) were...
Bordetella hinzii has recently been isolated from immunocompromised human hosts. The polysaccharides isolated from its endotoxin (lipopolysaccharide, LPS) were investigated using chemical analyses, NMR, gas-liquid chromatography/mass spectrometry and mass spectrometry by plasma desorption, matrix-assisted laser desorption/ionization and electrospray. The following structure for the O-chain-free LPS was deduced from the experimental results: carbohydrate structure [see text] Mass spectrometry and serology revealed that the O-chains were different from the homopolymer common to Bordetella bronchiseptica and Bordetella parapertussis strains and were composed of a trisaccharide repeating unit. Masses up to 8 kDa were obtained for native LPS molecular species.
Topics: Antibodies, Monoclonal; Bordetella; Carbohydrate Conformation; Carbohydrate Sequence; Chromatography, Thin Layer; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Gas Chromatography-Mass Spectrometry; Hydrolysis; Lipopolysaccharides; Magnetic Resonance Spectroscopy; Oligosaccharides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 11086162
DOI: 10.1016/s0014-5793(00)02181-5 -
Journal of Bacteriology Nov 2005Bordetella hinzii is a commensal respiratory microorganism in poultry but is increasingly being recognized as an opportunistic pathogen in immunocompromised humans.... (Comparative Study)
Comparative Study
Bordetella hinzii is a commensal respiratory microorganism in poultry but is increasingly being recognized as an opportunistic pathogen in immunocompromised humans. Although associated with a variety of disease states, practically nothing is known about the mechanisms employed by this bacterium. In this study, we show by DNA sequencing and reverse transcription-PCR that both commensal and clinical strains of B. hinzii possess and transcriptionally express cyaA, the gene encoding adenylate cyclase toxin (ACT) in other pathogenic Bordetella species. By Western blotting, we also found that B. hinzii produces full-length ACT protein in quantities that are comparable to those made by B. pertussis. In contrast to B. pertussis ACT, however, ACT from B. hinzii is less extractable from whole bacteria, nonhemolytic, has a 50-fold reduction in adenylate cyclase activity, and is unable to elevate cyclic AMP levels in host macrophages (nontoxic). The decrease in enzymatic activity is attributable, at least in part, to a decreased binding affinity of B. hinzii ACT for calmodulin, the eukaryotic activator of B. pertussis ACT. In addition, we demonstrate that the lack of intoxication by B. hinzii ACT may be due to the absence of expression of cyaC, the gene encoding the accessory protein required for the acylation of B. pertussis ACT. These results demonstrate the expression of ACT by B. hinzii and represent the first characterization of a potential virulence factor of this organism.
Topics: Adenylate Cyclase Toxin; Animals; Bacterial Proteins; Blotting, Western; Bordetella; Calmodulin; Cell Line; Cyclic AMP; DNA, Bacterial; Gene Expression; Hemolysis; Macrophages; Mice; Molecular Sequence Data; Protein Binding; RNA, Bacterial; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Virulence Factors, Bordetella
PubMed: 16267282
DOI: 10.1128/JB.187.22.7579-7588.2005 -
Comparative Medicine Oct 2008Bordetella hinzii isolated from the trachea and lungs of a laboratory mouse with a respiratory infection was identified based on its phenotypic and genetic traits. The...
Bordetella hinzii isolated from the trachea and lungs of a laboratory mouse with a respiratory infection was identified based on its phenotypic and genetic traits. The mouse showed sneezing with a chattering sound but without nasal discharge, and histopathologic examination revealed rhinitis, tracheitis, and bronchopneumonia. The isolate was a gram-negative, oxidase- and catalase-positive, short rod-shaped organism that produced alkali from malonate. The results of biochemical identification, an alkali production test from malonate, and partial sequence analysis of the 16S rRNA gene (1523 bp) were consistent with those reported previously for B. hinzii. The isolate induced sneezing in ICR mice and sneezing and slight to severe dyspnea in NOD-SCID mice after experimental infection. Histopathologic examination revealed catarrhal rhinitis and bronchopneumonia in both strains of mice and interstitial pneumonia in NOD-SCID mice. In light of these findings, B. hinzii was deemed to be a novel causative agent of respiratory disease in mice. This report describes the first isolation of B. hinzii from a mouse and confirms the organism's pathogenicity in mice.
Topics: Agglutination Tests; Animals; Bordetella; Bordetella Infections; DNA, Bacterial; DNA, Ribosomal; Female; Genotype; Lung; Mice; Mice, Inbred ICR; Mice, Inbred NOD; Mice, SCID; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Respiratory Tract Infections; Ribotyping; Rodent Diseases; Trachea
PubMed: 19004369
DOI: No ID Found -
Frontiers in Microbiology 2017Members of the genus include human and animal pathogens that cause a variety of respiratory infections, including whooping cough in humans. Despite the long known...
Members of the genus include human and animal pathogens that cause a variety of respiratory infections, including whooping cough in humans. Despite the long known ability to switch between a within-animal and an extra-host lifestyle under laboratory growth conditions, no extra-host niches of pathogenic species have been defined. To better understand the distribution of species in the environment, we probed the NCBI nucleotide database with the 16S ribosomal RNA (16S rRNA) gene sequences from pathogenic species. Bacteria of the genus were frequently found in soil, water, sediment, and plants. Phylogenetic analyses of their 16S rRNA gene sequences showed that recovered from environmental samples are evolutionarily ancestral to animal-associated species. Sequences from environmental samples had a significantly higher genetic diversity, were located closer to the root of the phylogenetic tree and were present in all 10 identified sequence clades, while only four sequence clades possessed animal-associated species. The pathogenic bordetellae appear to have evolved from ancestors in soil and/or water. We show that, despite being animal-adapted pathogens, , and have preserved the ability to grow and proliferate in soil. Our data implicate soil as a probable environmental origin of species, including the animal-pathogenic lineages. Soil may further constitute an environmental niche, allowing for persistence and dissemination of the bacterial pathogens. Spread of pathogenic bordetellae from an environmental reservoir such as soil may potentially explain their wide distribution as well as frequent disease outbreaks that start without an obvious infectious source.
PubMed: 28174558
DOI: 10.3389/fmicb.2017.00028 -
Genome Announcements Mar 2015Bordetella hinzii is a Gram-negative bacterium known to infect poultry, humans, rabbits, and rodents. It is an opportunistic pathogen in immunocompromised humans, and...
Bordetella hinzii is a Gram-negative bacterium known to infect poultry, humans, rabbits, and rodents. It is an opportunistic pathogen in immunocompromised humans, and some strains cause mild to moderate respiratory disease in turkeys. Little is known as to the degree of genetic diversity within the species or the genetic basis for virulence. Here, we report the genome sequences of six isolates of B. hinzii acquired from humans, rabbits, or turkeys. These data provide a framework for refining the population structure of the genus, establishing relationships among genetically distinct isolates, and developing an understanding of the possible virulence mechanisms of the bacterium.
PubMed: 25792043
DOI: 10.1128/genomeA.00081-15 -
International Journal of Systematic... Jan 1995A polyphasic taxonomic study that included DNA-rRNA hybridizations, DNA-DNA hybridizations, DNA base ratio determinations, whole-cell protein and fatty acid analyses,...
A polyphasic taxonomic study that included DNA-rRNA hybridizations, DNA-DNA hybridizations, DNA base ratio determinations, whole-cell protein and fatty acid analyses, and an examination of classical phenotypic characteristics was performed in order to classify human and veterinary isolates that resemble Bordetella avium. Twelve poultry isolates and two human isolates were assigned to a new species, for which we propose the name Bordetella hinzii. The position of this organism in the family Alcaligenaceae and various genotypic, phenotypic, and chemotaxonomic characteristics are described.
Topics: Animals; Bacterial Proteins; Bordetella; DNA, Bacterial; Electrophoresis, Polyacrylamide Gel; Fatty Acids; Humans; Poultry
PubMed: 7857806
DOI: 10.1099/00207713-45-1-37 -
Emerging Infectious Diseases Apr 2022Patients infected with severe acute respiratory syndrome coronavirus 2 might have bacterial and fungal superinfections develop. We describe a clinical case of...
Patients infected with severe acute respiratory syndrome coronavirus 2 might have bacterial and fungal superinfections develop. We describe a clinical case of coronavirus disease with pulmonary aspergillosis associated with Bordetella hinzii pneumonia in an immunocompetent patient in France. B. hinzii infections are rare in humans and develop secondary to immunosuppression or debilitating diseases.
Topics: Bordetella; COVID-19; Humans; Pneumonia; SARS-CoV-2
PubMed: 35318919
DOI: 10.3201/eid2804.212564 -
Journal of Clinical Microbiology Apr 2003Bordetella avium is an avian respiratory disease pathogen responsible for substantial economic losses to the turkey industry. The inability to distinguish isolates has... (Comparative Study)
Comparative Study
Bordetella avium is an avian respiratory disease pathogen responsible for substantial economic losses to the turkey industry. The inability to distinguish isolates has hampered outbreak investigations and prevents a complete understanding of transmission mechanisms. Isolates of Bordetella hinzii, often referred to as B. avium-like or as Alcaligenes faecalis type II prior to 1995, have also been acquired from the respiratory tracts of diseased poultry but are not believed to be pathogenic for birds. Therefore, differentiating between B. avium and B. hinzii is of importance for veterinary diagnostic laboratories. It was recently reported that both PvuII ribotyping and HinfI/DdeI restriction endonuclease analysis (REA) show promise for distinguishing isolates of B. avium and B. hinzii. Here we compare the ability of these techniques to discern inter- and intraspecies differences. While both approaches distinguished numerous types within a species, only REA was sufficiently discriminatory for routine use as an epidemiologic tool. Both techniques clearly distinguish between B. avium and B. hinzii, although the results of ribotyping are more easily interpreted. Ribotyping and REA identified numerous, previously unrecognized B. hinzii strains from a collection of bordetella isolates, including one acquired from a rabbit. This is the first report of B. hinzii isolation from a nonhuman mammalian species. At least some of the newly recognized B. hinzii isolates have been previously reported to cause disease in poults, suggesting that the pathogenicity of this agent for poultry should be more rigorously examined.
Topics: Animals; Bacterial Typing Techniques; Bordetella; Bordetella Infections; Chickens; DNA, Bacterial; DNA, Ribosomal; Ducks; Poultry Diseases; Rabbits; Restriction Mapping; Ribotyping; Species Specificity; Turkeys
PubMed: 12682138
DOI: 10.1128/JCM.41.4.1512-1519.2003 -
Neurology. Clinical Practice Oct 2021
PubMed: 34840911
DOI: 10.1212/CPJ.0000000000001122 -
Emerging Microbes & Infections Dec 2023
Topics: Humans; COVID-19; SARS-CoV-2; Bordetella; Bacteremia
PubMed: 36367206
DOI: 10.1080/22221751.2022.2147276